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Mix Carefully in advance of transferring cells to the conical tube that contains ten mL of pre-warmed clean medium. Be aware: Clean the pipette idea and cryovial Together with the clean medium no less than three instances to assist improve Restoration.

Circulation cytometry can be used to evaluate the efficiency of differentiation protocols While using the STEMdiff™ system, by taking a look at specific marker expression at Every single phase on the differentiation approach. It will also be utilized for other purposes, like cell sorting, immunophenotyping, and purity assessment.

If cells will not dissociate well with trituration, go with a longer ACCUTASE™ incubation, nearly twelve minutes. If monolayers do not dissociate just after twelve minutes of ACCUTASE™ incubation and trituration, the differentiation was possible unsuccessful.

The STEMdiff™ system gives a standardized technique for differentiating hPSCs into epithelial cells that could be afterwards cultured in 2D or 3D formats dependant upon the investigate requirements.

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Consider the consequences of take a look at compounds on hematopoietic stem cells with phenotypic and purposeful assessments.

Differentiation into adipocytes, osteoblasts, or chondrocytes with compatible MesenCult™ differentiation systems

Certainly, you’ll discover the measures to execute an ICC staining with your epithelial cells cultured Stem cell on the ALI Within this protocol. Here is an index of antibodies which can be utilized for the characterization of airway cultures:

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A least response Stem cell quantity of 50 µL is needed for regular efficiency While using the CellPore™ Transfection System.

Notice: PFA fixation may be viewed as for evaluating intracellular markers or undertaking Assessment at a later time.

Eliminate supernatant and resuspend cells in FACS buffer. Note: It is necessary to quench the dissociation reagent by using the similar or double the volume of the dissociation reagent.